LOCUS       40924_4734        5707 bp DNA     circular SYN 18-DEC-2018
DEFINITION  S. pombe expression vector pARG1H DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5707)
  AUTHORS   Matsuyama A, Shirai A, Yoshida M.
  TITLE     A novel series of vectors for chromosomal integration in fission 
            yeast
  JOURNAL   Biochem. Biophys. Res. Commun. 374 (2), 315-319 (2008)
  PUBMED    18634753
REFERENCE   2  (bases 1 to 5707)
  AUTHORS   Matsuyama A, Shirai A, Yoshida M.
  TITLE     Fission yeast integration vector pARG1H
  JOURNAL   Published Only in Database (2007)
REFERENCE   3  (bases 1 to 5707)
  AUTHORS   Matsuyama A, Shirai A, Yoshida M.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2007) Contact:Akihisa Matsuyama RIKEN, Chemical 
            Genetics Laboratory; Hirosawa 2-1, Wako, Saitama 351-0198, Japan URL
            :http://cgl.riken.go.jp/
REFERENCE   4  (bases 1 to 5707)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 5707)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biochem. 
            Biophys. Res. Commun."; date: "2008"; volume: "374"; issue: "2"; 
            pages: "315-319"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Published 
            Only in Database (2007)"
COMMENT     SGRef: number: 3; type: "Journal Article"; journalName: "Submitted 
            (15-OCT-2007) Contact:Akihisa Matsuyama RIKEN, Chemical Genetics 
            Laboratory; Hirosawa 2-1, Wako, Saitama 351-0198, Japan URL 
            :http://cgl.riken.go.jp/"
COMMENT     SGRef: number: 4; type: "Journal Article"
COMMENT     Fission yeast expression vector.
FEATURES             Location/Qualifiers
     source          1..5707
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      21..208
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae
                     alcohol dehydrogenase 1 (ADH1) gene"
     misc_feature    241..894
                     /pseudo
                     /gene="arg1"
                     /label=derived from Schizosaccharomyces pombe
                     /note="derived from Schizosaccharomyces pombe"
     CDS             241..894
                     /pseudo
                     /codon_start=1
                     /gene="arg1"
                     /label=arg1
                     /note="NotI site is created at the middle of this fragment 
                     (551-558)."
                     /translation="SRSC*R*GLLFV*QGRS*IH*LHLWRSCYIFGSCSS*SC*TCR*S
                     MLQISP**QPFLQRACH*AI*CH**FFGKK*RYCWPHKNFLCQLWY*GQ*DSLEVRS*G
                     GRV*EVWRREESNCLL**LFPRPFFGIS*YHCEPQI*TWFPTITS*CRPSRL**PCIHR
                     AICQ*QNCGCYCRARSR*RWYLSCQA*IFDCFAQGL**GWCFFNL**NSMRTWSQ"
     gene            241..894
                     /pseudo
                     /gene="arg1"
                     /label=arg1
     gene            938..2513
                     /label=hphMX6
                     /note="yeast selectable marker conferring hygromycin
                     resistance"
     primer_bind     complement(2567..2583)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      2796..3251
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        3533..3637
                     /label=AmpR promoter
     CDS             3638..4495
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      4669..5257
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    5545..5566
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        5581..5611
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5619..5635
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5643..5659
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 ggcgcgccac ttctaaataa gcgaatttct tatgatttat gatttttatt attaaataag
       61 ttataaaaaa aataagtgta tacaaatttt aaagtgactc ttaggtttta aaacgaaaat
      121 tcttattctt gagtaactct ttcctgtagg tcaggttgct ttctcaggta tagtatgagg
      181 tcgctcttat tgaccacacc tctaccggca gatccgctag ggataacagg gtaatatact
      241 agtcgtagct gctaaaggtg agggctccta tttgtttgac aaggaaggtc gtaaatacat
      301 tgacttcacc tctggcgtag ctgttacatc tttgggtcat gctcatcctg aagttgctag
      361 acttgccgct gatcaatgct ccaaattagt ccatagtagc aaccttttct acaacgagcc
      421 tgccattgag ctatctaatg tcattaataa ttctttggca aaaaatagcg gtattgctgg
      481 ccccacaaaa attttctttg ccaattgtgg tactgaggcc aatgagacag ccttgaagtt
      541 cgctcgtaag gcggccgcgt ttgagaagta tggagaaggg aagagtcaaa ttgtttactt
      601 taataactct ttccacggcc gttctttggg atctcttagt atcactgcga accccaaata
      661 taaacgtggt ttccaaccat tacttcctga tgtcgtccaa gccgtctata atgaccctgc
      721 atccatagag caatttgtca atgacaaaac tgcggctgtt attgtcgagc ccgttcaagg
      781 tgaaggtggt atttgtcctg ccaagcctga atttttgatt gctttgcgca aggcctgtga
      841 taaggttggt gcttctttaa tctatgatga aattcaatgc ggacttggtc gcagatctgt
      901 ttagcttgcc ttgtccccgc cgggtcaccc ggccagcgac atggaggccc agaataccct
      961 ccttgacagt cttgacgtgc gcagctcagg ggcatgatgt gactgtcgcc cgtacattta
     1021 gcccatacat ccccatgtat aatcatttgc atccatacat tttgatggcc gcacggcgcg
     1081 aagcaaaaat tacggctcct cgctgcagac ctgcgagcag ggaaacgctc ccctcacaga
     1141 cgcgttgaat tgtccccacg ccgcgcccct gtagagaaat ataaaaggtt aggatttgcc
     1201 actgaggttc ttctttcata tacttccttt taaaatcttg ctaggataca gttctcacat
     1261 cacatccgaa cataaacaac catgggtaaa aagcctgaac tcaccgcgac gtctgtcgag
     1321 aagtttctga tcgaaaagtt cgacagcgtc tccgacctga tgcagctctc ggagggcgaa
     1381 gaatctcgtg ctttcagctt cgatgtagga gggcgtggat atgtcctgcg ggtaaatagc
     1441 tgcgccgatg gtttctacaa agatcgttat gtttatcggc actttgcatc ggccgcgctc
     1501 ccgattccgg aagtgcttga cattggggaa ttcagcgaga gcctgaccta ttgcatctcc
     1561 cgccgtgcac agggtgtcac gttgcaagac ctgcctgaaa ccgaactgcc cgctgttctg
     1621 cagccggtcg cggaggccat ggatgcgatc gctgcggccg atcttagcca gacgagcggg
     1681 ttcggcccat tcggaccgca aggaatcggt caatacacta catggcgtga tttcatatgc
     1741 gcgattgctg atccccatgt gtatcactgg caaactgtga tggacgacac cgtcagtgcg
     1801 tccgtcgcgc aggctctcga tgagctgatg ctttgggccg aggactgccc cgaagtccgg
     1861 cacctcgtgc acgcggattt cggctccaac aatgtcctga cggacaatgg ccgcataaca
     1921 gcggtcattg actggagcga ggcgatgttc ggggattccc aatacgaggt cgccaacatc
     1981 ttcttctgga ggccgtggtt ggcttgtatg gagcagcaga cgcgctactt cgagcggagg
     2041 catccggagc ttgcaggatc gccgcggctc cgggcgtata tgctccgcat tggtcttgac
     2101 caactctatc agagcttggt tgacggcaat ttcgatgatg cagcttgggc gcagggtcga
     2161 tgcgacgcaa tcgtccgatc cggagccggg actgtcgggc gtacacaaat cgcccgcaga
     2221 agcgcggccg tctggaccga tggctgtgta gaagtactcg ccgatagtgg aaaccgacgc
     2281 cccagcactc gtccgagggc aaaggaataa tcagtactga caataaaaag attcttgttt
     2341 tcaagaactt gtcatttgta tagttttttt atattgtagt tgttctattt taatcaaatg
     2401 ttagcgtgat ttatattttt tttcgcctcg acatcatctg cccagatgcg aagttaagtg
     2461 cgcagaaagt aatatcatgc gtcaatcgta tgtgaatgct ggtcgctata ctgctgtcga
     2521 ttcgatacta acgccgccat ccagtgtcga aaacgagctc gaattcactg gccgtcgttt
     2581 tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc
     2641 cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt
     2701 tgcgcagcct gaatggcgaa tggcgcctga tgcggtattt tctccttacg catctgtgcg
     2761 gtatttcaca ccgcatacgt caaagcaacc atagtacgcg ccctgtagcg gcgcattaag
     2821 cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc
     2881 cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc
     2941 tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa
     3001 aaaacttgat ttgggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg
     3061 ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac
     3121 actcaaccct atctcgggct attcttttga tttataaggg attttgccga tttcggccta
     3181 ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac
     3241 gtttacaatt ttatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca
     3301 gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc
     3361 cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc
     3421 atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt
     3481 catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac
     3541 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
     3601 ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt
     3661 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct
     3721 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga
     3781 tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag
     3841 cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca
     3901 actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga
     3961 aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag
     4021 tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc
     4081 ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa
     4141 tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt
     4201 gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg
     4261 gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt
     4321 tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg
     4381 gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat
     4441 ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact
     4501 gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa
     4561 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
     4621 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     4681 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     4741 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     4801 gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     4861 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     4921 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     4981 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     5041 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     5101 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     5161 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     5221 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     5281 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
     5341 ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac
     5401 gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc
     5461 tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa
     5521 agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc
     5581 tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca
     5641 cacaggaaac agctatgacc atgattacgc caagcttgca tgcctgcagg tcgactctag
     5701 aggatcc
//
